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論文等による発表
2024
  1. Ryota Kurashiki, Masahiro Takahashi, Yuta Okumura, Tatsuya Ono, Hirofumi Endo, Kohei Makino, Kaho Fukui, Kyosuke Yokoyama, Shu Ishikawa, Ken-ichi Yoshida, Takashi Ohshiro, Hirokazu Suzuki. Efficient pathway-driven scyllo-inositol production from myo-inositol using thermophilic cells and mesophilic inositol dehydrogenases: a novel strategy for pathway control. Appl. Environ. Microbiol. 90, e0028124 (2024)
  2. Saori Kani, Hirokazu Suzuki. Whole genome sequence of Geobacillus thermodenitrificans K1041, a genetically tractable strain representative of the genus Geobacillus. Microbiol. Resour. Announc. 13, e0084823 (2024)
2023
  1. Ryota Kurashiki, Kosuke Koyama, Yukina Sakaguchi, Yuta Okumura, Takashi Ohshiro, Hirokazu Suzuki. Development of a thermophilic host-vector system for the production of recombinant proteins at elevated temperatures. Appl. Microbiol. Biotechnol. 107, 7475-7488 (2023)
2022
  1. Kosuke Koyama, Yui Mikawa, Shota Nakagawa, Ryota Kurashiki, Takashi Ohshiro, Hirokazu Suzuki. New platform for screening genetic libraries at elevated temperatures: biological and genomic information and genetic tools of Geobacillus thermodenitrificans K1041. Appl. Environ. Microbiol. 88, e0105122 (2022)
  2. 鈴木 宏和. 好熱菌における耐熱化酵素の発生と選択ならびに進化工学への応用. 化学工業 73, pp.634-640 (2022)
  3. Yoshihito Arai, Yunako Shingu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro. Occurrence of different fucoidanase genes in Flavobacterium sp. SW and enzyme characterization. J. Biosci. Bioeng. 134, 187-194 (2022)
2021
  1. 鈴木 宏和. 中等度好熱菌を利用してタンパク質をつくる. バイオインダストリー 11, pp.15-21 (2021)
  2. Takuto Shimizu, Emiko Nogami, Yuka Ito, Kazuo Morikawa, Masaki Nagane, Tadashi Yamashita, Tsuyoshi Ogawa, Fuyuki Kametani, Hisashi Yagi, Naomi Hachiya. Volatile anesthetic sevoflurane precursor 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) exerts an anti-prion activity in prion-infected culture cells. Neurochem. Res. 46, 2056-2065 (2021)
  3. Hirokazu Suzuki, Yuta Okumura, Yui Mikawa, Mao Takata, Shunsuke Yoshimura, Takashi Ohshiro. Transcriptome and growth efficiency comparisons of recombinant thermophiles that produce thermolabile and thermostable proteins: implications for burden-based selection of thermostable proteins. Extremophiles 25, 403-412 (2021)
  4. Hirokazu Suzuki, Tatsunari Taketani, Misaki Tanabiki, Misaki Ohara, Jyumpei Kobayashi, Takashi Ohshiro. Frequent transposition of multiple insertion sequences in Geobacillus kaustophilus HTA426. Front. Microbiol. 12, 650461 (2021)
2020
  1. Keisuke Wada, Hirokazu Suzuki. Biotechnological platforms of the moderate thermophiles, Geobacillus species: notable properties and genetic tools. In: Physiological and Biotechnological Aspects of Extremophiles (edited by Richa Salwan and Vivek Sharma), Elsevier, Amsterdam, Netherlands, pp.195-218 (2020)
  2. Ryota Kurashiki, Tatsuki Mizuno, Kurumi Murata, Takashi Ohshiro, Hirokazu Suzuki. A plasmid vector that directs hyperproduction of recombinant proteins in the thermophiles Geobacillus species. Extremophiles 24, 147-156 (2020)
2019
  1. 鈴木 宏和. 高変異性好熱菌を利用した耐熱化変異酵素のハイスループット創出. IFO Res. Commun. 33, pp.198 (2019)
  2. Keiko Fujimori, Satoru Fujii, Lisa Lindiana, Satoshi Wakai, Hisashi Yagi, Yoshihiro Sambongi. Differences in biochemical properties of two 5'-nucleotidases from deep- and shallow-sea Shewanella species under various harsh conditions. Biosci. Biotechnol. Biochem. 83, 1085-1093 (2019)
  3. Hirokazu Suzuki. Remarkable diversification of bacterial azoreductases: primary sequences, structures, substrates, physiological roles, and biotechnological applications. Appl. Microbiol. Biotechnol. 103, 3965-3978 (2019)
2018
  1. Norihito Uemura, Hisashi Yagi, Maiko T. Uemura, Yusuke Hatanaka, Hodaka Yamakado, Ryosuke Takahashi. Inoculation of α-synuclein preformed fibrils into the mouse gastrointestinal tract induces Lewy body-like aggregates in the brainstem via the vagus nerve. Mol. Neurodegener. 13, 21 (2018)
  2. 鈴木 宏和. 中等度好熱菌のスキマ研究を模索して. 鳥取大学工学部研究報告 49, pp.22-32 (2018)
  3. Hirokazu Suzuki. Peculiarities and biotechnological potential of environmental adaptation by Geobacillus species. Appl. Microbiol. Biotechnol. 102, 10425-10437 (2018)
  4. Hirokazu Suzuki, Tomoaki Abe, Katsumi Doi, Toshihisa Ohshima. Azoreductase from alkaliphilic Bacillus sp. AO1 catalyzes indigo reduction. Appl. Microbiol. Biotechnol. 102, 9171-9181 (2018)
  5. Tatsuhiko Nagao, Yoshihito Arai, Mika Yamaoka, Fumika Komatsu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro. Identification and characterization of the fucoidanase gene from Luteolibacter algae H18. J. Biosci. Bioeng. 126, 567-572 (2018)
  6. Kenta Fujii, Yurie Tominaga, Jyumpei Okunaka, Hisashi Yagi, Takashi Ohshiro, Hirokazu Suzuki. Microbial and genomic characterization of Geobacillus thermodenitrificans OS27, a marine thermophile that degrades diverse raw seaweeds. Appl. Microbiol. Biotechnol. 102, 4901-4913 (2018)
  7. Hisashi Yagi, Asako Fujise, Narumi Itabashi, Takashi Ohshiro. Characterization of a novel endo-type alginate lyase derived from Shewanella sp. YH1. J. Biochem. 163, 341-350 (2018)
  8. Hisashi Yagi, Natsuki Isobe, Narumi Itabashi, Asako Fujise, Takashi Ohshiro. Characterization of a long-lived alginate lyase derived from Shewanella species YH1. Mar. Drugs 16, 1-12 (2018)
  9. Hirokazu Suzuki, Tatsunari Taketani, Jyumpei Kobayashi, Takashi Ohshiro. Antibiotic resistance mutations induced in growing cells of Bacillus-related thermophiles. J. Antibiot. 71, 382-389 (2018)
2017
  1. Hirokazu Suzuki. Enzymatic strategies for cleaving glycosidic linkages. In: Advances in Chemistry Research (edited by James C. Taylor), Nova Science Publishers, New York, USA, vol.40, pp.173-196 (2017)
  2. Tatsuki Mizuno, Takashi Ohshiro, Hirokazu Suzuki. Plasmid curing is a promising approach to improve thermophiles for biotechnological applications: perspectives in archaea. In: Archaea - New Biocatalysts, Novel Pharmaceuticals and Various Biotechnological Applications (edited by Haitham Sghaier), InTech, Rijeka, Croatia, pp.83-99 (2017)
  3. Tomoya Hino, Haruka Hamamoto, Hirokazu Suzuki, Hisashi Yagi, Takashi Ohshiro, Shingo Nagano. Crystal structures of TdsC, a dibenzothiophene monooxygenase from the thermophile Paenibacillus sp. A11-2, reveal potential for expanding its substrate selectivity. J. Biol. Chem. 292, 15804-15813 (2017)
  4. Tatsuhiko Nagao, Ayako Kumabe, Fumika Komatsu, Hisashi Yagi, Hirokazu Suzuki, Takashi Ohshiro. Gene identification and characterization of fucoidan deacetylase for potential application to fucoidan degradation and diversification. J. Biosci. Bioeng. 124, 277-282 (2017)
  5. 鈴木 宏和. 耐熱化変異酵素を簡便かつ汎用的にスクリーニングする手法の開発. 公益財団法人長瀬化学技術財団研究報告集 28, pp.99-107 (2017)
  6. Jyumpei Kobayashi, Hirokazu Suzuki. Microbial responses and resistance to chloramphenicol. In: Advances in Medicine and Biology (edited by Leon V. Berhardt), Nova Science Publishers, New York, USA, vol.123, pp.53-91 (2017)
  7. Masahiko Okai, Woo Cheol Lee, Li-Jun Guan, Takashi Ohshiro, Yoshikazu Izumi, Masaru Tanokura. Crystal structure of dibenzothiophene sulfone monooxygenase BdsA from Bacillus subtilis WU-S2B. Proteins 85, 1171-1177 (2017)
  8. Hirokazu Suzuki. Geobacillus kaustophilus HTA426: a model organism for moderate thermophiles. In: Advances in Medicine and Biology (edited by Leon V. Berhardt), Nova Science Publishers, New York, USA, vol.114, pp.75-108 (2017)
2016
  1. 石野 園子，鈴木 宏和，河原林 裕. 極限環境微生物遺伝子資源の応用. IFO Res. Commun. 30, pp.103-117 (2016)
  2. Hisashi Yagi, Asako Fujise, Narumi Itabashi, Takashi Ohshiro. Purification and characterization of a novel alginate lyase from the marine bacterium Cobetia sp. NAP1 isolated from brown algae. Biosci. Biotechnol. Biochem. 80, 2338-2346 (2016)
  3. Yurie Tominaga, Takashi Ohshiro, Hirokazu Suzuki. Conjugative plasmid transfer from Escherichia coli is a versatile approach for genetic transformation of thermophilic Bacillus and Geobacillus species. Extremophiles 20, 375-381 (2016)
  4. Keisuke Wada, Jyumpei Kobayashi, Megumi Furukawa, Katsumi Doi, Takashi Ohshiro, Hirokazu Suzuki. A thiostrepton resistance gene and its mutants serve as selectable markers in Geobacillus kaustophilus HTA426. Biosci. Biotechnol. Biochem. 80, 368-375 (2016)
2015
  1. Li-Jun Guan, Woo Cheol Lee, Shipeng Wang, Takashi Ohshiro, Yoshikazu Izumi, Jun Ohtsuka, Masaru Tanokura. Crystal structures of apo-DszC and FMN-bound DszC from Rhodococcus erythropolis D-1. FEBS J. 282, 3126-3135 (2015)
  2. Jyumpei Kobayashi, Misaki Tanabiki, Shohei Doi, Akihiko Kondo, Takashi Ohshiro, Hirokazu Suzuki. Unique plasmids generated via pUC replicon mutagenesis in an error-prone thermophile derived from Geobacillus kaustophilus HTA426. Appl. Environ. Microbiol. 81, 7625-7632 (2015)
  3. Jyumpei Kobayashi, Megumi Furukawa, Takashi Ohshiro, Hirokazu Suzuki. Thermoadaptation-directed evolution of chloramphenicol acetyltransferase in an error-prone thermophile using improved procedures. Appl. Microbiol. Biotechnol. 99, 5563-5572 (2015)
  4. Jyumpei Kobayashi, Jotaro Yukimoto, Yasuhiro Shimizu, Taketo Ohmori, Hirokazu Suzuki, Katsumi Doi, Toshihisa Ohshima. Characterization of Lactobacillus salivarius alanine racemase: short-chain carboxylate-activation and the role of A131. SpringerPlus 4, 639 (2015)
  5. Hirokazu Suzuki, Jyumpei Kobayashi, Keisuke Wada, Megumi Furukawa, Katsumi Doi. Thermoadaptation-directed enzyme evolution in an error-prone thermophile derived from Geobacillus kaustophilus HTA426. Appl. Environ. Microbiol. 81, 149-158 (2015)
  6. Hirokazu Suzuki, Jun Ishii, Akihiko Kondo, and Ken-ichi Yoshida. Polyamino acid display on cell surfaces enhances salt and alcohol tolerance of Escherichia coli. Biotechnol. Lett. 37, 429-435 (2015)
  7. Hisashi Yagi, Aiko Mizuno, Masatomo So, Miki Hirano, Masayuki Adachi, Yoko Akazawa-Ogawa, Yoshihisa Hagihara, Tatsuya Ikenoue, Young-Ho Lee, Yasushi Kawata, Yuji Goto. Ultrasonication-dependent formation and degradation of α-synuclein amyloid fibrils. Biochim. Biophys. Acta 1854, 209-217 (2015)
  8. Mayu Terakawa-Suzuki, Hisashi Yagi, Masayuki Adachi, Young-Ho Lee, Yuji Goto. Small liposomes accelerate the fibrillation of amyloid β (1-40). J. Biol. Chem. 290, 815-826 (2015)
2014
  1. Takashi Ohshiro, Noriyuki Morita. Production and characterization of L-fucose dehydrogenase from newly isolated Acinetobacter sp. strain SA-134. Prep. Biochem. Biotechnol. 44, 382-391 (2014)
  2. Hironaga Akita, Yoshifumi Imaizumi, Hirokazu Suzuki, Katsumi Doi, Toshihisa Ohshima. Spectrophotometric assay of D-isoleucine using an artificially created D-amino acid dehydrogenase. Biotechnol. Lett. 36, 2245-2248 (2014)
  3. Hironaga Akita, Hirokazu Suzuki, Katsumi Doi, Toshihisa Ohshima. Efficient synthesis of D-branched-chain amino acids and their labeled compounds with stable isotopes using D-amino acid dehydrogenase. Appl. Microbiol. Biotechnol. 98, 1135-1143 (2014)
  4. Hirotsugu Ogi, Masahiko Fukukshima, Hiroki Hamada, Kentaro Noi, Masahiko Hirao, Hisashi Yagi, Yuji Goto. Ultrafast propagation of β-amyloid fibrils in oligomeric cloud. Sci. Rep. 4, 6960 (2014)
  5. Ayaka Umemoto, Hisashi Yagi, Masatomo So, Yuji Goto. High-throughput analysis of ultrasonication-forced amyloid fibrillation reveals the mechanism underlying the large fluctuation in the lag time. J. Biol. Chem. 289, 27290-27299 (2014)
  6. Cardine N. Nokwe, Martin Zacharias, Hisashi Yagi, Manuel Hora, Bernd Reif, Yuji Goto, Johannes Buchner. A residue-specific shift in stability and　amyloidogenicity of antibody variable domains. J. Biol. Chem. 289, 26829-26846 (2014)
  7. Hisashi Yagi, Yuki Abe, Naoto Takayanagi, Yuji Goto. Elongation of amyloid fibrils through lateral binding of monomers revealed by total internal reflection fluorescence microscopy. Biochim. Biophys. Acta 1844, 1881-1888 (2014)
  8. Jin Chen, Hisashi Yagi, Yuji Furutani, Takashi Nakamura, Asumi Inaguma, Hao Guo, Yan Kong, Yuji Goto. Self-assembly of the chaperonin GroEL nanocage induced at submicellar detergent. Sci. Rep. 4, 5614 (2014)
  9. Tatsuya Ikenoue, Young-Ho Lee, Jozsef Kardos, Miyu Saiki, Hisashi Yagi, Yasushi Kawata, Yuji Goto. Cold denaturation of α-synuclein amyloid fibrils. Angew. Chem. Int. Ed. Eng. 53, 7799-7804 (2014)
  10. Hiroya Muta, Young-Ho Lee, Jozsef Kardos, Yuxi Lin, Hisashi Yagi, Yuji Goto. Supersaturation-limited amyloid fibrillation of insulin revealed by ultrasonication. J. Biol. Chem. 289, 18228-18238 (2014)
  11. Tatsuya Ikenoue, Young-Ho Lee, Jozsef Kardos, Hisashi Yagi, Takahisa Ikegami, Hironobu Naiki, Yuji Goto. Heat of supersaturation-limited amyloid burst directly monitored by isothermal titration calorimetry. Proc. Natl. Acad. Sci. USA. 109, 6654-6659 (2014)
  12. Yuxi Lin, Young-Ho Lee, Yuichi Yoshimura, Hisashi Yagi, Yuji Goto. Solubility and supersaturation-dependent protein misfolding revealed by ultrasonication. Langmuir, 1845-1854 (2014)
  13. 鈴木 宏和，小林 淳平. 耐熱化酵素を好熱菌細胞内で創る． 化学と生物 52 pp.428-430 (2014).
  14. Hirokazu Suzuki, Jyumpei Kobayashi, Keisuke Wada, Megumi Furukawa, Katsumi Doi. Generation of thermostable enzyme genes using spontaneous mutations in thermophile Geobacillus kaustophilus HTA426. Industrial, Medical and Environmental Applications of Microorganisms: Current Status and Trends (edited by A. Mendez-Vilas), Wageningen Academic Publishers, Wageningen, Netherlands, pp.441-446 (2014)
  15. Jyumpei Kobayashi, Misaki Tanabiki, Megumi Furukawa, Hisashi Yagi, Takashi Ohshiro, Hirokazu Suzuki. Generation of chloramphenicol resistance plasmids functional at high temperatures by using an error-prone thermophile. Conference Proceedings of International Biotechnology, Chemical Engineering and Life Science Conference 2014 (IBCELC), pp.66-73 (2014)
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